Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Grp75 Monoclonal Antibody, Clone S52A-42 . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Grp75 Monoclonal Antibody  at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondria. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Grp75 Antibody. (C) Composite.