Broad sarcolemmal distribution of compensatory proteins upon Akt activation. Immunohistochemical analyses on transverse quadriceps sections in WT STG, WT DTG, mdx STG and mdx DTG mice. Sections were stained with antibodies to dystrophin (Dys), utrophin (Utrn), β1D integrin, <t>alpha-</t> and beta-dystroglycan (α-DG, β-DG), alpha-, beta- and <t>gamma-sarcoglycan</t> (α-SG, β-SG, γ-SG) and sarcospan (SSPN), and visualized using indirect immunofluorescence. Increased expression of the DGC and UGC in WT mice was observed upon constitutive activation of Akt1. Akt activation increased expression of only the UGC in mdx mice. An increase in utrophin levels was observed in mdx mice relative to levels in WT mice. In both WT mice and in mdx mice, Akt activation increased the expression of β1D integrin. Bar, 50 µm. 